HPLC Operation and Troubleshooting Training Course

Name: HPLC Operation, Troubleshooting and Maintenance Training Course
Availability: InStock

Operate HPLC systems, verify analytical performance, isolate chromatographic faults, and plan controlled maintenance.

HPLC Operation and Troubleshooting Training Course

At a glance

Duration: 1 Week
Format: Classroom
Cities: Muscat, Tbilisi, Bangkok, Baku, Berlin, Trabzon and more
Next session: 15 – 19 November 2026, Muscat
Average fee: 7,500 €

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HPLC Operation, Troubleshooting and Maintenance Training Course

Overview

HPLC Operation, Troubleshooting and Maintenance Training Course is a one-week intermediate course for laboratory analysts, quality-control chemists, pharmaceutical analysts, and technical support staff who leave with an HPLC Reliability and Analysis File. The course connects HPLC system operation, chromatographic method setup, sample and mobile-phase preparation, system suitability testing, instrument calibration, HPLC troubleshooting, preventive maintenance, and chromatography data integrity across pharmaceutical and industrial laboratories. Agile Leaders Training Center delivers this course on practical HPLC reliability.

Who Should Attend

Teams responsible for preparing samples, standards, mobile phases, and analytical sequences
Functions responsible for operating pumps, injectors, columns, detectors, and chromatography software
Teams responsible for system suitability, calibration checks, result review, and data integrity
Functions responsible for diagnosing pressure, baseline, peak-shape, retention, and carryover problems
Technical groups responsible for preventive maintenance, service records, and return-to-use decisions

The course assumes participants already follow laboratory procedures and interpret basic chromatograms, and leaves out instrument repair authorization, software administration, and method validation approval.

Departments and Industries

The course supports controlled HPLC work across laboratory, manufacturing, environmental, and research settings.

Pharmaceutical quality control, analytical development, and stability testing
Food, beverage, chemical, and materials testing laboratories
Environmental monitoring, water analysis, and public-health laboratories
Research, biotechnology, and contract analytical services
Laboratory engineering, metrology, maintenance, and quality assurance

Learning Objectives

By the end of this course, participants will be able to:

Analyze HPLC flow paths and chromatographic separation behavior.
Apply controlled sample and mobile-phase preparation methods.
Use system suitability tests and calibration evidence.
Diagnose pressure, baseline, retention, peak-shape, and carryover faults.
Build preventive maintenance and data-integrity controls.
Evaluate corrective action and return-to-use evidence.

Course Agenda

Day 1: Map HPLC Operation

HPLC Separation Mechanisms and Reversed-Phase Selectivity Map
Solvent Reservoir, Degasser, Pump, Mixer, and Flow-Path Diagram
Autosampler, Injection Volume, Needle-Wash, and Carryover Control Checklist
Analytical Column Chemistry, Particle Size, Temperature, and Pressure Selection Matrix
Ultraviolet Detector, Signal Acquisition, Integration, and Chromatogram Review Method

Day 2: Prepare and Configure Analysis

Reference Standard, Sample Dilution, Filtration, and Stability Preparation Record
Mobile-Phase Composition, Buffer, pH, Degassing, and Compatibility Worksheet
Isocratic and Gradient Elution Parameter Setup Sheet
Sequence Table, Blank, Standard, Sample, Wash, and Reinjection Design
Retention Factor, Selectivity, Resolution, Efficiency, and Tailing Calculation Set

Day 3: Verify System Performance

USP <621> Chromatography System Suitability Decision Table
Flow-Rate, Injection-Precision, Detector-Response, and Wavelength Check Record
Calibration Status, Performance Qualification, and Traceability Evidence Review
Control Sample, Bracketing Standard, Drift, and Acceptance Trend Chart
Raw Data, Audit Trail, Integration Change, and Chromatography Data Integrity Checklist

Day 4: Diagnose and Maintain HPLC

High Pressure, Low Pressure, Leakage, and Flow Instability Fault Tree
Baseline Noise, Drift, Spikes, and Detector Contamination Diagnostic Matrix
Peak Tailing, Fronting, Splitting, Broadening, and Retention Shift Investigation
Ghost Peak, Carryover, Solvent Mismatch, and Contamination Isolation Sequence
Pump Seal, Check Valve, Injector, Detector Cell, Column, and Tubing Maintenance Plan

Day 5: Practice HPLC Decisions

Exercise: Reconcile Flow Path, Pressure, Signal, and Chromatogram Evidence
Exercise: Prepare Sample, Mobile Phase, Sequence, and Suitability Controls
Exercise: Isolate Baseline, Peak-Shape, Retention, and Carryover Causes
Exercise: Evaluate Maintenance Action and Return-to-Use Evidence
Capstone Exercise: Build the HPLC Reliability and Analysis File

Practical Exercises

The course uses suggested activities to connect chromatographic evidence with controlled laboratory decisions.

Suggested activity: trace the HPLC flow path and match pressure or signal symptoms to likely components.
Suggested activity: calculate suitability parameters and decide whether an analytical sequence can proceed.
Suggested activity: investigate a changed chromatogram using preparation, method, column, instrument, and data evidence.
Suggested activity: assemble maintenance findings, corrective actions, verification results, and return-to-use criteria.

FAQs

Who suits HPLC operation, troubleshooting and maintenance training, and what does it assume?

HPLC operation, troubleshooting and maintenance training suits laboratory and quality-control personnel who already follow analytical procedures and interpret basic chromatograms.

How does HPLC troubleshooting differ from analytical method development?

HPLC troubleshooting isolates causes of unexpected pressure, baseline, retention, peak-shape, or carryover behavior, while analytical method development selects and optimizes separation conditions for a defined analytical purpose.

What causes unstable pressure during HPLC system operation?

Unstable HPLC pressure can result from air, leaks, restricted tubing or frits, contaminated check valves, solvent incompatibility, pump-seal wear, or a partially blocked column.

What evidence supports HPLC return-to-use after maintenance?

HPLC return-to-use requires resolved fault causes, acceptable leak and flow checks, stable detector response, passing suitability results, controlled maintenance records, and documented review of the resulting chromatograms.

Conclusion

Participants take back an HPLC Reliability and Analysis File. The file connects preparation records, method settings, suitability calculations, calibration evidence, diagnostic findings, maintenance actions, data reviews, and return-to-use criteria. It changes isolated chromatographic symptoms into a traceable basis for analysis control, fault diagnosis, preventive care, and defensible laboratory decisions.

credits: 5 credit per day

Course Mode: full-time

Provider: Agile Leaders Training Center

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Showing 1-20 of 58 events

Location	Dates	Duration	Mode	Price
Muscat	Week 46, 2026 15 – 19 November 2026	1 Week	Onsite	€6,500
Tbilisi	Week 47, 2026 16 – 20 November 2026	1 Week	Onsite	€5,700
Bangkok	Week 47, 2026 22 – 26 November 2026	1 Week	Onsite	€8,000
Baku	Week 49, 2026 30 November – 4 December 2026	1 Week	Onsite	€8,000
Berlin	Week 49, 2026 30 November – 4 December 2026	1 Week	Onsite	€6,500
Trabzon	Week 49, 2026 6 – 10 December 2026	1 Week	Onsite	€8,000
Abu Dhabi	Week 50, 2026 7 – 11 December 2026	1 Week	Onsite	€6,500
Cape town	Week 50, 2026 13 – 17 December 2026	1 Week	Onsite	€6,000
Casablanca	Week 52, 2026 21 – 25 December 2026	1 Week	Onsite	€6,000
London	Week 01, 2027 4 – 8 January 2027	1 Week	Onsite	€6,500
Amman	Week 01, 2027 10 – 14 January 2027	1 Week	Onsite	€6,000
Jakarta	Week 03, 2027 18 – 22 January 2027	1 Week	Onsite	€8,000
Frankfurt	Week 03, 2027 18 – 22 January 2027	1 Week	Onsite	€6,500
Dubai	Week 05, 2027 1 – 5 February 2027	1 Week	Onsite	€6,500
Zanzibar	Week 05, 2027 7 – 11 February 2027	1 Week	Onsite	€6,000
Tokyo	Week 07, 2027 15 – 19 February 2027	1 Week	Onsite	€12,000
Madrid	Week 09, 2027 1 – 5 March 2027	1 Week	Onsite	€6,500
Addis Ababa	Week 09, 2027 1 – 5 March 2027	1 Week	Onsite	€6,000
Kuwait	Week 09, 2027 7 – 11 March 2027	1 Week	Onsite	€7,000
Kuala Lumpur	Week 11, 2027 15 – 19 March 2027	1 Week	Onsite	€6,500

Frequently asked questions

What does this course cover?

OverviewHPLC Operation, Troubleshooting and Maintenance Training Course is a one-week intermediate course for laboratory analysts, quality-control chemists, pharmaceutical analysts, and technical support staff who leave with an HPLC Reliability and Analysis File. The course connects HPLC system operation, chromatographic method setup, sample and mobile-ph…

Are training dates available?

Yes. Available dates and destinations are listed in the course dates section on this page.

How can I register?

Choose an available date on this page and complete the registration form, or send a programme enquiry.

Can I download the course brochure?

Yes. Use the brochure download link provided on this page.

HPLC Operation and Troubleshooting Training Course